November 18th

Lexie W
November 18th, 2010
Scribe



Agenda: Protein Synthesis Lab
Homework: Tribune Article (Due Monday)
Finish Lab Questions (for tomorrow)



In Mr. Paek’s 4th period class today we did a lab on Protein Synthesis.

In this activity we:
- understand the roles of mRNA and tRNA in protein synthesis
- construct a model of protein synthesis
It again goes though the DNA bases and their pairings:
Guanine→Cytosine
Adenine→Thymine
The packet also shows RNA bases and pairings:
Guanine→Cytosine
Adenine→Uracil

The materials were in the nucleic acid model kit.
Cytosine (blue)
Thymine (green)
Adenine (orange)
Guanine (yellow)
Phosphates (white tubes)
Hydrogen Bonds (white rods)
Deoxyribose (black pentagon)
Uracil (purple)
Ribose sugar (purple pentagon)
Peptide bonds (grey)
tRNA grouper
Amino Acid with R-groups

The first step we had to do was build a double stranded DNA molecule with:
T T T C C A T G C as the left hand strand.

Right strand would be: A A A G G T A G C

Then we “unzipped” the DNA molecule into the two strands

Using the left strand of the DNA molecule as the master strand. And we then constructed a matching mRNA strand. (using the ribose and uracil base)
A A A G G U A C G

That is the process of transcription.
The mRNA sequence was the same as the right hand side except for the one Uracil base.

We then unzipped the mRNA strand from the DNA strand.
Later, we made three tRNA molecules by taking one of the tRNA molecules and placing three bases on it that matched the first three nucleotides of the mRNA strand. These are the anitcodons.

Anticodons that were constructed:
AAA, GGU, ACG

We then attached an amino acid to the correct tRNA molecule by it R-group. We then attached amino acid and this tRNA complex to the codon of mRNA with hydrogen bonds. The amino acids were connected through peptide bonds between them. This is a polypeptide chain.

THE NEXT SCRIBER IS…………………….
CONNOR! ☺ revenge is sweet ☺

i give dambi permission to write whatever she wants here because she is posting this for me :)

I LOVE LEXIE!!!!!!!!!-dambi